Study on Tissue Culture of Purple Potato by Tissue Culture Room

Anthocyanins have many physiological functions such as prevention of cardiovascular and cerebrovascular diseases, inhibition of tumor cell formation, etc. Purple potatoes contain a large amount of this substance, while purple potatoes are vegetatively propagated crops and are susceptible to viral infection and degenerative during cultivation. As the seed potato carries virus, the disease will be severe, which will lead to a reduction of 10% to 30%, and a serious 70%. The sterile potato plants cultivated by detoxification through the stem tip can preserve the long-term productive potential of the superior varieties, and the establishment of a series of tissue culture and rapid propagation systems can provide excellent and high-yielding seed potatoes for production. Culture experiments were conducted using a tissue culture chamber.

Potato tubers were either dormant or soaked with l% thiourea + 5 mg/l gibberellin for 5 minutes to break dormancy. The dormant tubers were removed and buried with sterile moist fine sand. When the top buds of the tubers grew to 3 to 4 cm, they were pretreated at a high temperature of 36°C to passivate the virus. Then, cut the top 2 to 3 cm buds, remove all the leaves, and wash thoroughly with tap water. Place it in a beaker and cover it with gauze for 30 minutes. Soak in 70% alcohol for 30 seconds on the clean bench, wash once with sterile water, then sterilize with 0.1% HgCl2 for 5-8 minutes, and finally wash with sterile water 5 times and place in a closed sterile bottle. In standby. The buds that can be disinfected once should not be too much, so as to avoid long time for the materials to be placed after sterilization, browning of the stem tips, and affecting the survival rate.

On the clean bench, under a 40-fold double-dissection dissection microscope, carefully remove the small leaves around the shoot tip with a scalpel, and then remove the leaf primordium with an anatomical needle until the top of the leafy growth point is exposed. Carefully cut the desired stem. Apical meristems, and finally only the growth point size is 0.1 to 0.2 mm. After being cut down, it was inoculated on the potato shoot tip culture medium, and the agar was cut in sections. Each tube was inoculated with 1 stem tip, and the sealed bottle was placed in a tissue culture room for ex vivo culture. The culture flasks are preferably 15 cm x 2 cm test tubes, each containing 10 ml of culture medium. After inoculation, the inoculated cultivar and the date of inoculation are marked on the paper cap outside the upper end of the test tube.

The cultivated cells in the tissue culture room were observed for cultivation of the treated potato, and found that after treatment of detoxification at the shoot tip, rapid propagation of tissue culture, and induction of detoxified minitubers, breeding of the original species and improved varieties was carried out until the production of commodity potatoes in Datian. It is the best way to significantly increase the yield and quality of purple potato by combining the stem tip detoxification technology and high-efficiency seed-retaining technology, and establishing a scientific and reasonable breeding system.

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